Pharmacological Assays of Plant-Based Natural Products

1. Extraction of plant material -- 2. Critical aspects of purification and impurities in natural product extracts. 3. In vitro antioxidant assays -- 4. In vivo antioxidant assays -- 5. Quantification assays -- 6. Anti-nutritional factors -- 7. Anti-inflammatory activity -- 8. Antipyretic activity -- 9. Inflammation bowel disease -- 10. Anti-arthritic activity -- 11. Evaluation of analgesic potential -- 12. Anti-diarrhoeal activity -- 13. Anti-ulcer activity -- 14. In vitro anti-diabetic activity -- 15. In vivo anti-diabetic activity -- 16. Hepatoprotective property -- 17. Antitumor activity -- 18. In vivo wound healing studies -- 19. Determination of cytotoxicity -- 20. Evaluation of bioavailability -- 21. Molecular identification by DNA barcoding -- 22. Molecular docking of bioactive compounds against BRCA and COX proteins.;This volume provides information on how to select and screen plants for their medicinal properties. It describes phytopharmacological techniques for extracting and qualitatively and quantitatively analyzing a plantâ#x80;#x99;s phytochemicals. After a detailed in vitro investigation including nutritional and anti-nutritional analyses, medicinal properties were tested with various in vivo models for anti-inflammatory, analgesic, anti-pyretic, anticancer and anti-diabetic properties, as well as wound healing, neurodegenerative diseases, etc. Compound identification and purification techniques include, among others, TLC and column chromatography, as well as molecular docking with specific proteins.